Isolation, identification and antibiotic susceptibility profiles in bacterial strains isolated from periodontal lesions

The dental plaque biofilm is at origin of the pathogenic mechanisms of the periodontal lesions, resulting both from the direct degradative action and the indirect inflammatory lesions, mediated by microbial cells and their virulence factors. The purpose of this study was the isolation, identification and characterization of the antibiotic susceptibility profiles of bacterial strains isolated from patients with periodontal lesions. The identification of the 54 isolated bacterial strains was based on the examination of colonial, culture, microscopic and biochemical features. The obtained data show a large taxonomic diversity of the bacterial strains isolated from pacients with periodontal lesions (30 species), the most frequent being Actinomyces neslundii (12.96%), Pasteurella haemolytica (11.11%), Micrococcus sp. (7.4%) and Streptococcus intermedius (7.4%). The antibiotic susceptibility assay revealed different profiles and significant levels of antimicrobial resistance, reflecting the necessity to perform the microbiological analysis and the antibiotic susceptibility testing in order to select the optimal antimicrobial therapy for the treatment of the periodontal disease.